Polymerase Cain Reaction (PCR) yog cov txheej txheem tseem ceeb hauv cov roj ntsha biology. Cov ntim ntawm nws cov reagents thiab cov khoom ua ncaj qha cuam tshuam cuam tshuam kev ntseeg tau ntawm kev sim thiab kuaj kom muaj kev ruaj khov. Txheem prc cov txheej txheem ntim khoom siv xav tau kev tswj hwm nruj nruj dhau ntawm kev xaiv cov khoom xaiv, kev paug kev tiv thaiv, thiab daim ntawv labeling.
Xaiv cov khoom ntim yog qhov tseem ceeb. PCR cov tshuaj tiv thaiv cov tshuab hluav taws xob xws li cov khoom siv DNA xws li DNA qauv, cov ntawv tso cai, enzymes, thiab buffers. Siab - - free (dnase} PUB DAWB (DNEATE} PUB DAWB Ntim ntim yuav tsum kaw kom nruj kom tsis txhob muaj cov kua los yog evaporation. Cov lus qhia pipette pipette raug pom zoo kom txo qis kev pheej hmoo ntawm aerosol tsis sib thooj.
Anti {- ntsuas ntsuas yog qhov tseem ceeb heev. PCR yog qhov raug rau tsis tseeb - Qhov txiaj ntsig zoo tau vim muaj kev paug los ntawm Diagenous DNA. Yog li ntawd, ntim yuav tsum tau ua nyob rau hauv ib qho chaw huv (xws li lub rooj huv huv). Cov neeg ua haujlwm yuav tsum hnav cov hnab looj tes ntawm hnab looj tes, lub ntsej muag, thiab lub tsho ntev thiab lwm yam uas yuav tsum tau hloov pauv tsis tu ncua. Different samples or reagents should be packaged separately to prevent cross-contamination. Rau kev lag luam pcr cov khoom siv, cov chaw tsim khoom muag cov qauv tsim nyog ntim cov qauv ntim yuav tsum tau ua raws. Kev tsis siv qhov pib yuav tsum tau muab ntim thiab muab khaws cia ntawm -20 degree, tiv thaiv los ntawm lub teeb, thiab tiv thaiv los ntawm kev rov ua kom khov thiab yaj.
Sau npe thiab cov qauv khaws cia yog qhov tseem ceeb. Txhua lub ntim ntim yuav tsum muaj daim ntawv cim qhia meej nrog tus qauv npe, concentration, hnub tim thiab cov neeg teb xov tooj kom tsis txhob muaj kev ntxhov siab. Fluorescent kom muaj nuj nqi psgents yuav tsum muab cia ntawm lub teeb thiab ntim rau txhuas ntawv ci hnab lossis tsaus} xim tub. Tsis tas li ntawd, cov ntaub ntawv ntim khoom ntim yuav tsum muab cov khoom siv sib txawv raws li lawv cov yam ntxwv: piv txwv li, cov enzymes yuav tsum tau muab xa tawm ntawm chav tsev kub tab sis yuav tsum tau txais kev tiv thaiv los ntawm noo noo.
Hauv cov ntsiab lus, qhov qauv ntawm pcr ntim tau ncaj qha cuam tshuam nrog qhov tseeb ntawm cov ntaub ntawv sim. Dhau ntawm cov ntaub ntawv xaiv nruj, ib puag ncig tswj, thiab cov kev pheej hmoo ntawm kev ua kom tsawg, ua kom muaj kev phom sij thiab kev tshawb fawb ntawm kev sim ua ntej. Kev cob qhia cov neeg saib xyuas yuav tsum muaj kev cob qhia tsis tu ncua los txhawb kev paub txog cov txheej txheem ntim qauv qauv.